This study was undertaken to investigate the effects of chemical agents on clonorchis sinensis and host´s liver tissues.
In vitro, flukes were tested with ethyl alcohol-ethyl ether solution of different concentration.
The experimental rabbits were infected each with 500 of metacercariae and 35 to 40 days later a polyethylene catheter of gauge No. 20 was inserted into the common bile duct of each rabbit, then ethyl alcohol-ethyl ether solution was infused through the catheter.
Fifty days after infection, the rabbits were sacrificed to study the histopathological changes of the liver and to detect the infected flukes in the liver, then the following results were obtained.
1. The flukes were killed within 10 seconds in the original solution (one part of 99% ethyl alcohol plus two parts of ethyl ether), 2 to 5 minutes in 50°o soluton diluted with 0.9% NaCl solution and 5 to 10 minutes in 25% solution in vitro.
2. The reduction in number of infected flukes in the ethyl alcohol-ethyl ether infusion group was 63% in average compared to the control group.
3. Necrosis and abscess of the liver parenchyma, inflammation of the bile ducts and the vessels, dilatation of the blood vessels and cirrhotic changes were more marked in the group of ethyl alcohol-ethyl ether infusion than those seen in the other groups and directly related to the concentration of the solution.
4. The degree of the hepatic enlargement was generally proportional to the. histopath -ological changes of the liver and inversely proportional to the body weight of the rabbits.
5. One shot of infusion with concentrated ethyl alcohol-ethyl ether solution, namely the original solution was superior to the low concentrated solution of multiple infusion for elimination of the infected flukes and induced less damages to the liver.
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